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- Paediatric Cancers
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Abstract
Citation: Clin Oncol. 2022;7(1):1931.DOI: 10.25107/2474-1663.1931
MTT Cytotoxicity Study of Extracts of Meyna laxiflora Seeds and Tectona grandis Bark using Cell Lines LLC PK1
Sunita S Deore, Amar G Zalte and Vishal S Gulecha
School of Pharmaceutical Sciences, Sandip University, Nashik, India
*Correspondance to: Sunita S Deore
PDF Full Text Short Communication | Open Access
Abstract:
The LLC-PK1 (Sus scrofa kidney epithelial cell line) is purchased from ATCC, USA. The cells were maintained in DMEM high glucose media supplemented with 10% FBS along with the 1% antibiotic-antimycotic solution in the atmosphere of 5% CO2, 18 to 20% O2 at 37°C temperature in the CO2 incubator and sub cultured for every 2 days.
Keywords:
Cytotoxicity; Cell lines; DMEM; Lactate dehydrogenase; Diaphorase; Tetrazolium salt
Cite the Article:
Deore SS, Zalte AG, Gulecha VS. MTT Cytotoxicity Study of Extracts of Meyna laxiflora Seeds and Tectona grandis Bark using Cell Lines LLC PK1. Clin Oncol. 2022;7:1931..