MTT Cytotoxicity Study of Extracts of Meyna laxiflora Seeds and Tectona grandis Bark using Cell Lines LLC PK1

Sunita S Deore, Amar G Zalte and Vishal S Gulecha

School of Pharmaceutical Sciences, Sandip University, Nashik, India

^*Correspondance to: Sunita S Deore 

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Abstract:

The LLC-PK1 (Sus scrofa kidney epithelial cell line) is purchased from ATCC, USA. The cells were maintained in DMEM high glucose media supplemented with 10% FBS along with the 1% antibiotic-antimycotic solution in the atmosphere of 5% CO₂, 18 to 20% O₂ at 37°C temperature in the CO₂ incubator and sub cultured for every 2 days.

Keywords:

Cytotoxicity; Cell lines; DMEM; Lactate dehydrogenase; Diaphorase; Tetrazolium salt

Cite the Article:

Deore SS, Zalte AG, Gulecha VS. MTT Cytotoxicity Study of Extracts of Meyna laxiflora Seeds and Tectona grandis Bark using Cell Lines LLC PK1. Clin Oncol. 2022;7:1931..