Huanhuan Shi, Chengcheng LV and Yunfeng Zhu*
Department of Life Sciences and Bio-Engineering, Beijing Jiao-tong University, PR ChinaFulltext PDF
Objective: To investigate the mechanism of Grape Seed Proanthocyanidins (GSPs) on colon cancer cells.Methods: SW480 and SW620 cells were treated with GSPs and characterized by evaluating cell morphology, cell viability, cell cycle and apoptosis respectively. The expression and activation of some important molecules were explored by PCR and Western blot respectively.Results: GSPs suppressed the proliferation and altered the morphology of both SW480 and SW620 cells significantly for long term treatment. GSPs caused arrest of SW480 and SW620 cell cycle in G0/G1 phase and facilitated apoptosis. Cyclin D1 and CDK4 as well as some molecules in Akt signaling pathway were identified to be down-regulated by GSPs. Furthermore, GSPs also suppressed the activation of Akt and some downstream molecules.
Grape seed proanthocyanidins; Colorectal cancer; Apoptosis
Shi H, Chengcheng LV, Zhu Y. Grape Seed Proanthocyanidins Alters the Characteristics of Colon Cancer Cells Regarding an Akt Signaling. Clin Oncol. 2019; 4: 1602.