Differences in the Molecular Landscape of Colorectal Cancer in Old and Young Patients

Kozlowska-Geller M, Strzelecka A, Nawacki L, Wawszczak-Kasza M, Gluszek S and Lewitowicz P

Medical College, The Jan Kochanowski University in Kielce

^*Correspondance to: Monika Kozlowska-Geller 

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Abstract:

Introduction: Colorectal cancer is a major global public health issue, especially in Western countries. The application of Next-Generation Sequencing (NGS) technology has facilitated multigene panel analysis and is used to identify individuals with cancer-predisposing gene variants. Aim: We analyzed the molecular differences in colorectal cancer in patients younger than 50 years and older than 50 years. Material and Methods: To the inclusion criteria We used no previous radio- or chemotherapy or DNA eligible for next-generation sequencing. The patients were divided into two groups: up to 50 years of age and over 50 years of age. DNA was isolated from FFPE tissue. We used a hot spot cancer panel (Illumina) harboring 50 genes (700 amplicons). Results: The median age of the young and older patients was 43 and 63 years, respectively. The female-to-male ratio was 1.2:1. We observed a following mutation frequency (≤ 50/>50): TP53 76/64%, APC 57/45%, KRAS 43/73%, NRAS 29/0%, SMAD4 9/15%, PIK3CA 14/33%, FBXW7 5/15%. We noted the co-occurrence APC/KRAS/TP53 mutation in 20% of patients. KRAS mutations were significantly more common in elderly patients (p=0.001). Conclusion: Almost half of the respondents (46%) presented multigene abnormalities, with three to five or more mutations being the most common. IDH1 and CTBX1 are found only in patients with a better prognosis when the TP53 mutation is almost twice as common in patients with worse prognosis. Interestingly is the fact that 29% of the young have NRAS mutations, the old do not have it at all, p=0.021.

Keywords:

Genomic era; Next-Generation-Sequencing (NGS); Personalized medicine

Cite the Article:

Kozlowska-Geller M, Strzelecka A, Nawacki L, Wawszczak-Kasza M, Gluszek S and Lewitowicz P. Differences in the Molecular Landscape of Colorectal Cancer in Old and Young Patients. Clin Oncol. 2024;9:2106..