Analysis of lncRNA-miRNA-mRNA and circRNA-miRNAmRNA ceRNA Network in Oral Squamous Cell Carcinoma

Xuying L, Kun Z, Jincun L, Wen D, Chen H, Dongyang L, Jian M

Department of Oral and Maxillofacial Surgery, General Hospital of Ningxia Medical University

^*Correspondance to: Ma Jian 

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Abstract:

Background: Increasing evidence suggests that long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) function as competitive endogenous RNAs (ceRNAs) by sponging microRNAs (miRNAs), thereby influencing the expression of target genes. The ceRNA networks involving lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA interactions have been implicated in various cancers, yet their specific roles in Oral Squamous Cell Carcinoma (OSCC) remain unclear. Methods: OSCC and corresponding paracancerous tissue samples were collected from three patients undergoing surgical resection. RNA expression profiles were obtained using an Illumina high-throughput sequencing platform. Differential Expression analysis identified significantly altered lncRNAs (DElncRNAs), miRNAs (DEmiRNAs), and mRNAs (DEmRNAs) between OSCC and paracancerous tissues using the limma and DESeq packages in R. We constructed lncRNAmiRNA- mRNA and circRNA-miRNA-mRNA ceRNA networks based on miRanda-predicted interactions using Cytoscape (version 3.10.0). Functional enrichment analysis including Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was performed using the ClusterProfiler package in R, with visualization using ggplot2. Results: We identified 1188 DElncRNAs, 281 circRNAs, 170 DEmiRNAs, and 4663 DEmRNAs in OSCC. The OSCC-specific lncRNA-miRNA-mRNA ceRNA network comprised 299 nodes and 512 edges (219 DElncRNA-DEmiRNA interactions and 293 DEmiRNA-DEmRNA interactions). The OSCC-specific circRNA-miRNA-mRNA ceRNA network consisted of 12 nodes and 11 edges (8 DEcircRNA-DEmiRNA interactions and 3 DEmiRNA-DEmRNA interactions). Conclusion: Our study provides insights into the regulatory mechanisms underlying OSCC progression. We propose lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA ceRNA networks that may facilitate further exploration of OSCC molecular mechanisms.

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Cite the Article:

Xuying L, Kun Z, Jincun L, Wen D, Chen H, Dongyang L, Jian M. Analysis of lncRNA-miRNA-mRNA and circRNA-miRNAmRNA ceRNA Network in Oral Squamous Cell Carcinoma. Clin Oncol. 2024;9:2102..