Adipose-Derived Mesenchymal Stem Cells Modified by PTEN Gene Can Specifically Target Tumor Cells

Chen M, Bi H, Du J, Yang C, Li H, Shao J, Fu Y, Liu H, He X, He L, Guo X, Wang H, Jin Z and Li Q

Department of Pulmonary and Critical Care Medicine, Affiliated Calmette Hospital of Kunming Medical University and the First People's Hospital of Kunming City, China
Department of Respiratory and Critical Care Medicine, Shanghai East Hospital, Tongji University School of Medicine, China
These authors contributed equally to this work

^*Correspondance to: Zhixian Jin 

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Abstract:

The Phosphatase and Tensin Homolog detected on chromosome ten (PTEN) is a tumor suppressor gene with double phosphatase activity. Injection of PTEN alone for the treatment of Non-Small Cell Lung Cancer (NSCLC) often has side effects. In this study, Adipose-Derived Mesenchymal Stem Cells (ADSCs) from ICR mice were selected as cell carriers to carry the PTEN gene for NSCLC therapy. Lentiviral transfection technology (PTENADSCs) was used to transfect the PTEN gene into ADSCs. LLC cells were subcutaneously injected into mice to establish NSCLC mouse models. After removing 50 adjacent tissues and 50 cancerous tissues, 30 mice were randomly divided into treatment group (ADSCs and PTEN-ADSCs) and non-treatment group. Gene differential expression analysis was performed on paracancerous tissue and cancer tissue, and ADSCs and PTEN-ADSCs were injected into LCC lung cancer mice in the treatment group, respectively. Cells in cancer tissue were disorganized and nested. Congestion and necrosis and disappearance of cell morphology were found in the tumor area, and apoptosis was significantly lower than that in the adjacent tissues. The protein expression level of PTEN in cancer tissue was significantly lower than that in caspase-3, bcl-2, ERK1, MAPK, PI3K, MMP-2 and MMP-9, and the relative expression level of PTEN mRNA was significantly higher than that in the adjacent tissues. Immunohistochemical staining also showed that PTEN was low expression in cancer tissue. The tumor growth rate of ADSCs and PTEN-ADSCs in the treatment group was significantly smaller than that of the nontreatment group, and both of them could inhibit the growth of NSCLC tumors, but the inhibition rate of tumor growth in the PTEN-ADSCs group was significantly higher than that in the ADSCs group. PTEN-ADSCs can specifically target tumor cells and reduce the damage to internal organs when PTEN is used alone, and the specific mechanism of action needs to be further studied.

Keywords:

PTEN; ADSCs; Non-small cell lung cancer; Tumorigenicity

Cite the Article:

Chen M, Bi H, Du J, Yang C, Li H, Shao J, et al. Adipose-Derived Mesenchymal Stem Cells Modified by PTEN Gene Can Specifically Target Tumor Cells. Clin Oncol. 2024;9:2094..