Sonodynamic Treatment Combined with PEITC Effects on C6 Glioma Cells In Vitro

Kong CC, Kan ZS, Fu L, Sun XY, Zhang F, Da Si MH, Mai Ti M, Wei X, Peng JJ and Li JH

Department of Gynecology and Obstetrics, The First College Hospital of Haerbin Medical University, China
Department of Neurosurgery, Cancer Hospital, Chinese Academy of Medical Sciences, China

^*Correspondance to: Jian-hua Li 

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Abstract:

The aim of the study was hematoporphyrin monomethyl ether-mediated sonodynamic therapy combined with PEITC to kill C6 cells. Methods: In the SDT+PEITC group, C6 cells received acoustic power treatment at an intensity of 0.5 W/cm2 and a frequency of 1 MHz, followed by incubated in 10 μM of the ITCs was used for 6 h. Tumor inhibition rate was assessed by MTT method and apoptosis rate was assessed by flow cytometry. Reactive Oxygen Species (ROS) and mitochondrial depolarization were detected by applying their respective methods. Additionally, cleavage of caspase-3 and caspase-9 was investigated through immunoblotting. Results: In SDT+PEITC, the tumor suppression rate was significantly higher than that in the SDT or PEITC alone group. And, the apoptosis rate in the SDT+PEITC group was the highest. Additionally, the SDT+PEITC group exhibited higher levels of ROS generation and mitochondrial depolarization compared to the SDT or PEITC alone groups. Conclusion: Based on our findings, it can be concluded that the combined treatment of SDT and PEITC, known as SDT+PEITC, exerts a potent cytotoxic effect on C6 glioma cells in vitro through synergistic mechanisms.

Keywords:

C6 glioma cells; Apoptosis; SDT+PEITC (Sonodynamic Therapy combining with Phenethyl Isothiocyanate); Reactive Oxygen Species (ROS)

Cite the Article:

Kong CC, Kan ZS, Fu L, Sun XY, Zhang F, Da Si MH, et al. Sonodynamic Treatment Combined with PEITC Effects on C6 Glioma Cells In Vitro. Clin Oncol. 2023;8:2043..