OCT4 Affects Gastric Cancer Progression by Promoting Proliferation, Migration, and Inhibiting Differentiation

Zirong Pan

Department of General Surgery, Xiamen Haicang Hospital, China

^*Correspondance to: Zirong Pan 

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Abstract:

Background: The Octamer-binding transcription factor 4 (OCT4), a driving factor widely involves in cellular pluripotency of cancer stem-like cells, the roles in cell differentiation became an increasing interest nowadays. Methods: The mRNA expression of OCT4 in Gastric Cancer (GC) tissues or GC cell lines were determined by Gene Expression Profiling Interactive Analysis (GEPIA) database or quantitative reverse transcription PCR (qRT-PCR). Survival analysis of OCT4 was also identified by Kaplan- Meier plotter. Further, the relative protein expression of OCT4 and BMP-SMAD pathway in GC cell lines was analyzed using Western blot. Functionally, cell proliferation, migration, and differentiation were detected by Cell Counting Kit-8 (CCK-8), 5-Ethynyl-2’-Deoxyuridine (EDU) assay, Wound healing assay and Western blot, respectively. In addition, tumor xenograft model was established to investigate this OCT4 functions in vivo. Results: In this study, high-expressed OCT4 was found in GC tissues and GC cell lines and exhibited a poor prognosis. Functionally, knockdown of OCT4 inhibited cell proliferation and migration in HGC-27 cells, whereas overexpressed OCT4 facilitated cell proliferation and migration in MKN-28 cells. The mRNA expression of CD133 and E-cadherin were increased in HGC-27 and MKN-28 cells silencing OCT4. It was found that OCT4 silencing stimulated a decrease on the tumor weight and volume as compared with control group in vivo. HE staining and immunohistochemistry suggested that knockdown of OCT4 promoted tumor differentiation. On the mechanism, BMPSMAD signaling-related genes expression changed abnormally in HGC-27 cells. Conclusion: OCT4 could promote cell proliferation, migration and differentiation, which was involved in BMP-SMAD signaling in human GC.

Keywords:

OCT4; Gastric cancer; Proliferation; Migration; Cell differentiation

Cite the Article:

Zirong Pan. OCT4 Affects Gastric Cancer Progression by Promoting Proliferation, Migration, and Inhibiting Differentiation. Clin Oncol. 2023;8:2022..