Effects of Vialinins A and B on Murine Splenocytes Sensitized with Ovalbumin

Jun-ichi Onose, Fumiyo Sekiya, Aki Shiomitsu, Yasukiyo Yoshioka, Kouichi Sugaya and Naoki Abe

Department of Nutritional Science and Food Safety, Faculty of Applied Bio-science, Tokyo University of Agriculture, Japan

^*Correspondance to: Naoki Abe 

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Abstract:

Vialinins A and B, as strong inhibitors of the production and release of tumor necrosis factor (TNF)-α, have been isolated from the edible Chinese mush room, Thelephoravialis. Here we investigated the inhibitory effects of vialinins A and B on the immune system. Splenocytes obtained from ovalbumin (OVA)-sensitized BALB/c mice were challenged with OVA in the presence of vialinins A and B, and cytokine levels in the medium of cultured cells were measured. Vialinins A and B inhibited production of OVA-specific immunoglobulin (Ig)E and Th2-type cytokines (interleukin (IL)-4, IL-5, and IL-10) but not production ofTh1-type cytokines (interferon-γ, IL-2, and IL-12). Flow cytometric assay showed a significantly higher percentage of regulatory T cells (CD25- and Foxp3-positive T cells) among splenocytes cultured with OVA and vialinins A and B than among those cultured with OVA alone. This offers a first demonstration that vialinins A and B inhibit antigen-specific IgE- and Th2-type cytokines and regulation of regulatory T cells, suggesting the utility of vialinins A and B in preventing deleterious immune responses.

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Cite the Article:

Jun-ichi Onose, Sekiya F, Shiomitsu A, Yoshioka Y, Sugaya K, Abe N. Effects of Vialinins A and B on Murine Splenocytes Sensitized with Ovalbumin. Clin Oncol. 2017; 2: 1357.