Transcriptional Levels of PRUNE-1 are Correlated to Prognostic Parameters in Epithelial Tumors

Larissa Vargas, Sara TS Mota, Sarah BR Nunes, Fabrícia M Oliveira, Galber R Araujo, Adriana F Neves, Yara CP Maia, Luiz Ricardo Goulart and Thaise G Araújo

Department of Biochemistry, Federal University of Uberlândia, Brazil
Department of Mathematics, Federal University of Uberlândia, Brazil
Department of Genetics and Biochemistry, Federal University of Uberlândia, Brazil
Department of Laboratory of Molecular Biology, Federal University of Goiás GO, Brazil
Department of Medicine, Federal University of Uberlândia, Brazil

^*Correspondance to: Thaise G Araujo 

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Abstract:

Background: The human orthologue of Drosophila PRUNE protein (PRUNE-1) regulates cell motility through its phosphodiesterase activity and interaction with protein partners, acting as a negative regulator of Nm23-H1, a tumor suppressor protein. Here, we aimed to understand the molecular alterations in epithelial tumors through quantification of PRUNE-1 transcriptional levels in patients with Prostate Cancer (PCa) and Breast Cancer (BC).Methods: mRNA expression was detected by RT-qPCR in peripheral blood from 31 PCa, 30 Benign Prostatic Hyperplasia BPH, 48 Breast Cancer, and 41 Benign Breast Disease patients.Results: Transcriptional levels of h-PRUNE in PCa were associated to high grade of Gleason score. Regression analyses had shown correlation between Prostate Specific Antigen, Gleason and PRUNE-1 mRNA expression. In BC, transcripts were significantly higher in HER-2 overexpression group compared to luminal patients.Conclusion: Up-regulation profile of PRUNE-1 may indicate convergent pathways for progression and aggressive PCa and BC. Our results pointed PRUNE-1 as a putative gene for identify metastasis.

Keywords:

Epithelial tumors; PRUNE-1; Transcription; Metastasis

Cite the Article:

Vargas L, Mota STS, Nunes SBR, Oliveira FM, Araujo GR, Neves AF, et al. Transcriptional Levels of PRUNE-1 are Correlated to Prognostic Parameters in Epithelial Tumors. Clin Oncol. 2017; 2: 1327.