Plasma Albumin Alterations in Gastrointestinal Cancer Patients

Inta Kalnina, Tija Zvagule and Jelena Kirilova

Department of Chemistry, Daugavpils University, Latvia
Department of Chemistry, RJga Stradins University, Latvia

^*Correspondance to: Inta Kalnina 

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Abstract:

Albumin plays a prime role in most functional characteristics of plasma and immune state of organism. Patients with gastrointestinal cancer exhibit a poorly functioning immune system. This study work investigated the possibility of using the fluorescent probe ABM for detecting structural and functional alterations of blood plasma albumin in pathological states. The aim of this research work was to evaluate the changes of blood albumin in correlation with some immunological parameters in patients with gastrointestinal cancer. 46 patients with gastrointestinal cancer and 24 healthy donors were examined in this study. Lymphocyte subpopulations were determined by flow cytometry using the appropriate specific monoclonal antibodies in the peripheral blood. Patients were tested one day before the operation and ten days after surgical treatment. The fluorescent probe - amino derivative of benzanthrone, ABM (developed at Daugavpils University, Daugavpils, Latvia) was used to characterize the blood plasma albumin. Before surgical treatments, the average ABM intensity in the patients' blood plasma was decreased compared to that seen with the samples from the healthy donors. The lymphocyte distribution among subsets differed not only between observed groups of patients, but also before and after surgical treatment. ABM fluorescence intensity was found to correlate with the absolute number of CD38+ cells and with some immunological parameters both before and after surgical treatment. To conclude, measures of ABM fluorescence values for lymphocytes and blood plasma could potentially be used in clinical immunological screening (instead of more expensive routine test) to provide a snapshot of immune status of individuals.

Keywords:

Blood plasma albumin; Immune diagnostics; Fluorescent probe ABM; Gastrointestinal cancer; Lymphocytes

Cite the Article:

Kalnina I, Zvagule T, Kirilova J. Plasma Albumin Alterations in Gastrointestinal Cancer Patients. Clin Oncol. 2016; 1: 1113.