Caixia Chen1, Hui Gao2 and Xiulan Su1*
1Clinical Medicine Research Center, The Key Laboratory of Biological Cells of Inner Mongolia Autonomous Region, The Affiliated Hospital, Inner Mongolia Medical University, China
2Department of Thoracic Surgery, Inner Mongolia Autonomous Region Cancer Hospital, China
Background: Bioactive Hepatic Peptide (BHP), a kind of bioactive peptide from goat liver, can regulate immune function in healthy mice. However, how the tumor-bearing mice? We investigated the effect of BHP on the immune function of gastric cancer-bearing nude mice and its possible underlying mechanism.
Methods: Nude mice were inoculated into the neck with human BGC-823 gastric cancer cells to establish the subcutaneous transplanted tumor model group. The percentage of peripheral blood lymphocytes was measured by flow cytometry. Hematoxylin-Eosin (HE) staining was used. The mRNA expression levels were measured by real-time fluorescence quantitative PCR and the protein expression of NF-κB, TNFα, Beclin1, LC3B, Akt1, COX-2 were determined by immunohistochemistry and Enzyme-Linked Immunosorbent Assay (ELISA).
Results: BHP could inhibit tumor cell proliferation, improve the percentage of NK cells/B lymphocytes, phagocytic index and phagocytic percentage and reduce the degree of inflammatory infiltration in tumor-bearing nude mice. BHP could significantly decrease the mRNA and protein expression of NF-κB, TNF-α in spleen and AKT1 and COX-2 in tumor tissue, but it could
significantly increase the mRNA and protein expression of Beclin1 and LC3B (LC3) in tumor tissue (P<0.05). The levels of NF- κB, TNFα and COX-2 were significantly lower in the serum of nude mice treated with BHP (P<0.05). The level of Beclin1 increased significantly after BHP treatment (P<0.05).
Conclusion: BHP may down regulate NF-κB/AKT1 to inhibit inflammation and induce autophagy, thus enhancing the immune function and antitumor effect of tumor-bearing nude mice. These findings support BHP as an immune-boosting health food/drug for cancer patients.
Bioactive Hepatic Peptide (BHP); Immunity; Inflammation; Autophagy
Chen C, Gao H, Su X. Bioactive Hepatic Peptide Improves Immune Function by Inducing Autophagy in Stomach Cancer-Bearing Nude Mice. Clin Oncol. 2021;6:1851..