Hiroaki Ishibashi1*, Naotshi Sugimoto2, Sousuke Yamada3, Shinichiro Sumitomo1 and Yasunori Muramatsu1
1Department of Oral and Maxillofacial Surgery, Asahi University, Japan
2Department of Pathology and Laboratory Medicine, Kanazawa Medical University, Japan
3Department of Physiology, Kanazawa University, Japan
The effects of inhibition of transcription factor AP-1 on synthesis of urokinase type Plasminogen Activator (uPA), uPA Receptor (uPAR), and Vascular Endothelial Growth Factor (VEGF) were determined using cultured salivary gland carcinoma cells (ACCS and ACCT cells). The expression of uPA, uPAR and VEGF was induced in the presence of EGF. To inhibit AP-1 activation, we
transfected double-stranded synthetic oligonucleotides containing the binding sequence of AP-1 (AP-1 decoy ODNs) using a novel, hemagglutinating virus of Japan-, liposome method. Transfection of AP-1 decoy ODNs into ACCS and ACCT cells inhibited EGF-induced uPA and uPAR mRNA expression. AP-1 decoy ODN transfection reduced the in vitro invasion activity of both cells in the presence of EGF to 27.0 and 39.4%, respectively. EGF-induced mRNA expression of VEGF was simultaneously suppressed by AP-1 decoy ODNs. Inhibition of AP-1 by decoy ODNs has potential as a novel method for the simultaneous inhibition of salivary gland carcinoma cell invasion and angiogenesis.
Salivary gland carcinoma; Urokinase type plasminogen activator; Urokinase type plasminogen activator receptor; Vascular endothelial growth factor; AP-1 decoy
Ishibashi H, Sugimoto N, Yamada S, Sumitomo S, Muramatsu Y. Transfection of Cis-Element ‘Decoy’ AP-1 DNA Binding Sequences Inhibits Expression of uPA, uPAR and VEGF in Salivary Gland Cancer Cells. Clin Oncol. 2021;6:1849..