Minchao Duan1* and Caixia Qin2
1Department of Respiratory Medicine, Wuming Hospital of Guangxi Medical University, China
2Department of Respiratory Medicine, The Eighth People’s Hospital of Nanning, China
Objective: Tc17 cells and Interleukin-17 (IL-17) are involved in the development of lung cancer. IL-27 has been suggested as a heterodimeric cytokine for influencing T cell activities. However, their distributions, biological function as well as the relationship are still lacking. In this study, we investigated the expression of IL-27 and its association with Tc17 cells in Non-Small Cell Lung
Cancer (NSCLC) patients.
Methods: Using flow cytometry, qPCR, and ELISA, the frequency of Tc17 cells and the expression of IL-27 and IL-17 from peripheral blood, tumor tissue, and peritumoral tissue of NSCLC patients and from peripheral blood of healthy controls were evaluated. The relationships among Tc17 cells, IL-27, and IL-17 were analyzed.
Results: The frequency of Tc17 cells and the expression of IL-27 were markedly decreased in peripheral blood from NSCLC patients compared with that in controls; the expression of IL-17A protein and mRNA in serum was markedly increased in NSCLC patients. In addition, the frequency of Tc17 cells was higher in peritumoral tissues than in tumor specimens and peripheral blood.
Whereas the expression of IL-17 and IL-27 was higher in tumor specimens than those in peritumoral tissues and corresponding serum. Furthermore, the expression of IL-27 in tumor specimens and peripheral blood was positively correlated with Tc17 cells numbers, but not IL-17 expression. Conclusion: Collectively, IL-27, IL-17, and Tc17 cells are involved in NSCLC immunity and elicit protumor or anti-tumor respectively.
Interleukin-27; Interleukin-17; Tc17 cell; Inflammation; Non-small cell lung cancer
Duan M, Qin C. The Expression and Relationship of Tc17 Cells and Interleukin-27 in Local and Systemic Environment of Non-small Cell Lung Cancer. Clin Oncol. 2021;6:1806..