Anula Divyash Singh1,3, Sreenu B2, Syed Baseeruddin Alvi3, Sreekanth Patnam1,3, Rajeswari K1, Vijay Kumar Kutala2, Aravind Kumar Rengan3 and Manda Sasidhar Venkata1,4*
1Apollo Hospitals Educational and Research Foundation (AHERF), India
2Nizam's Institute of Medical Sciences, Hyderabad, India (NIMS), India
3Department of Biomedical Engineering, Indian Institute of Technology Hyderabad (IITH), India
4Urvogelbio Private Ltd, India
Curcumin is a promising anti-cancer drug with limited aqueous solubility and decreased bioavailability. Milk Extracellular Vesicles (MEVs) used as a drug-delivery vehicle, were precipitated with Poly-Ethylene-Glycol (PEG) MW3000 from milk-whey. The yield of MEVs was 200.0 ± 0.85 mg/liter of whey. MEVs were spherical, double-membrane structures with a size range of 92 ± 30
nm/Nanoparticle-Tracking-Analysis (NTA) and 87 ± 40 nm/Dynamic-Light Scattering (DLS). A 70% loading efficiency was achieved with a passive loading of curcumin. The mean size of mevcurcumin was 152 ± 49 nm/NTA and 113 ± 67 nm/DLS. A six-fold increase of cytotoxicity and a two fold increase in curcumin accumulation were observed by mev-curcumin over native curcumin in
MDA-MB231, a breast cancer cell-line. Thus, we report the development of a curcumin formulation with improved solubility, stability, and cellular permeability for breast-cancer treatments.
Singh AD, Sreenu B, Alvi SB, Patnam S, Rajeswari K, Kutala VK, et al. Bovine Milk Derived Exosomal - Curcumin Exhibiting Enhanced Stability, Solubility, and Cellular Bioavailability. Clin Oncol. 2021;6:1769..