Ayyub A Patel1, Mohammed Amanullah1*, Mohamed Eissa2, Fahmy G Elsaid3 and Tamer Soliman4
1Department of Clinical Biochemistry, King Khalid University, KSA 2Department of Pathology, King Khalid University, KSA; Department of Clinical Pathology, Zagazig University, Egypt 3Department of Biology, College of Science, King Khalid University, KSA; Department of Zoology, Mansoura University, Egypt 4Department of Clinical Pathology, Sohag University, EgyptFulltext PDF
The cytotoxicity of Saussurea lappa (Asteraceae) aqueous extract was examined on five different cell lines; Human Lung Fibroblast Cells (MRC5), Human Dermal Fibroblast adult (HDFa), Breast Cancer Cells (MCF7), Human Colonic Cancer (Caco2) and Canine Kidney cells (MDCK). Saussurea lappa extract showed cytotoxic activities with IC50 values ranging from 2.5 mg/ml to 0.85 mg/ ml and it was observed that the Human Lung Fibroblast cells were the most sensitive cells (IC50 values; 0.8). The genotoxic potentialities of extract were observed as regulatory mechanism of gene expression with the finding that Saussurea lappa extract could regulate cell apoptosis. In MCF7 treated cells, treatments had the ability to down regulate the expression of both P53 and Bcl2 genes, whereas TGF, BAX, IKaB were up regulated. On the other hand, the treated Caco2 cells showed down regulation for P53, Bcl2 and IKaB genes.
Saussurea lappa; Colon cancer; Breast cancer; Dermal fibroblasts; Genotoxic potentialities
Patel AA, Amanullah M, Eissa M, Elsaid FG, Soliman T. A Study on Anti-Cancer Properties of Saussurea lappa (Asteraceae) Against Breast and Colonic Cancer Cell Lines. Clin Oncol. 2020; 5: 1702.