Ceren Gonen-Korkmaz1*, Mehmet Zuhuri Arun1, Buket Reel1, Lokman Varıslı2, Mazen Saeed Abdulaziz1 and Gokce YILDIRIM-BUHARALIOGLU1
1Department of Pharmacology, Ege University, Bornova, Izmir, Turkey
2Department of Bioengineering, Cancer Biology Laboratory, Ege University, Bornova, Izmir, Turkey
Epigenetic investigates gene expression modifications not due to DNA sequence alterations. Gene expression modifications appear with packing of DNA with variety of chromatin structures. The most studied forms of epigenetic phenomenon are DNA methylation and histone modifications. These pathways are connected with each other and reversible. DNA methylation reported genes: 36B4 (as control), HN1 primers were used and methylation status had been confirmed at DU145 cell line. Histone modifications which close the gene expression is histone deacetylation had been reversed by Trichostatin A (TSA). It is reported that inhibition of methylation and deacetylation in a serial way result with successful gene activation, performed with 5 Azacitidine and TSA incubation at the project. In the recent years, epigenetic base had been shown for many diseases that research and development for epigenetic disorders won successful treatment cases. Preclinical and clinic levels of candidate drugs are on the way
HN1; DU145; TSA; 5-Azasitid
Gonen-Korkmaz C, Arun MZ, Reel B, Var?sl? L, Abdulaziz MS, YILDIRIMBUHARALIOGLU G. Epigenetic Regulation of Hematological and Neurological Expressed 1(HN-1) at DU145 Brain Metastases Prostate Cancer Cell Line. Clin Oncol. 2017; 2: 1314.